Journal: Bioengineering (Basel, Switzerland)

Article Title: Manufacturing and Functional Characterization of Bioengineered Liver Grafts for Extracorporeal Liver Assistance in Acute Liver Failure.

doi: 10.3390/bioengineering10101201

Figure Lengend Snippet: Figure 2. Cellular identification and histology of BELs. HUVECs and PHLCs were characterized prior to seeding and within the graft. HUVECs expression of CD31 and CD105 was evaluated by flow cytometry prior to seeding into the decellularized graft. (A) Representative histogram of HUVEC expression of endothelial markers. (B) Percentage of HUVECs stained positive for CD31 and CD105 from Figure 1A (n = 25). (C) Glucose consumption rate during the endothelial culture period of the decellularized scaffold. The shaded area depicts two standard deviations from the mean. (D) Flow Cytometry scatter profile of PHLC population. Hepatocytes are indicated based on size and complexity by gated area. Gating is representative. (E) ASGR expression within a representative PHLC population. Gate indicates positive expression as compared to appropriate isotype control. The population shown is representative of the PHLC population after isolation. (F) Hepatocyte quantification within the BEL population as determined from the scatter plot gating strategy. (n = 14) (G) H&E showing a cross-section of a BEL recellularized with HUVECs and PHLCs one-day post hepatocyte seeding. (H) H&E showing a cross-section of a HUVEC lined vessel in a BEL recellularized with HUVECs and PHLCs. Arrows show representative areas of HUVEC-lined vessels. (I) H&E showing a highly magnified cross-section of a BEL recellularized with HUVECs and PHLCs hepatocytes one-day post hepatocyte seeding. Arrows show HUVECs lining the vessel.

Article Snippet: Cells were fixed using 2% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) and stained for CD31 (BIORAD, Hercules, CA, USA #MCA1738; 1:100 dilution) and CD105 (BIORAD, Hercules, CA, USA #MCA1557; 1:100 dilution) expression.

Techniques: Expressing, Cytometry, Staining, Flow Cytometry, Control, Isolation

Journal: eLife

Article Title: Differential chondrogenic differentiation between iPSC derived from healthy and OA cartilage is associated with changes in epigenetic regulation and metabolic transcriptomic signatures

doi: 10.7554/eLife.83138

Figure Lengend Snippet: ( A ) The morphology of the iMSC-like cells (iPSC–MSC) derived from AC- and OA-iPSC showing elongated spindle-shaped cells. Representative images are shown for iMSCs at passages 5–8. Scale bar, 100 μm. Image displays representative experiment ( n = 3). ( B ) Gene expression analyses by qPCR showing significant suppression of pluripotent markers OCT4 and SOX2, and induction of mesenchymal genes TWIST1, COL1A1, and RUNX1 in the AC- and OA-iMSCs relative to their parental iPSCs. β-Actin served as the housekeeping gene and internal control. Results from one representative experiment ( n = 3). Expression data are represented as fold change relative to respective parental iPSCs. *p ≤ 0.01, as compared to their respective iPSCs. ( C ) Expression of surface antigens in AC- and OA-iMSCs by flow analysis. Representative flow cytometric histogram showing OA-iMSCs (#2) express markers associated with the mesenchymal phenotype (positive for CD44, CD73, CD90, CD105, and CD166; negative for CD31 and CD45) ( n = 3). Red histogram shows antibody-stained population; blue profile shows negative isotype-stained population. ( D ) Comparative flow cytometry analyses of AC-iMSCs (#7, #14, and #15) and the OA-iMSCs (#2, #5, and #8) showing similar cell surface expression profiles. Results from one representative experiment ( n = 3). Figure 2—source data 1. Depicting original raw data related to . Figure 2—source data 2. Table providing data related to .

Article Snippet: Antibody , FITC Mouse Anti-Human CD105, Mouse monoclonal , BD-Biosciences , 561443, RRID: AB_10714629 , 1:100 for flow cytometry.

Techniques: Derivative Assay, Expressing, Staining, Flow Cytometry

Journal: eLife

Article Title: Differential chondrogenic differentiation between iPSC derived from healthy and OA cartilage is associated with changes in epigenetic regulation and metabolic transcriptomic signatures

doi: 10.7554/eLife.83138

Figure Lengend Snippet:

Article Snippet: Antibody , FITC Mouse Anti-Human CD105, Mouse monoclonal , BD-Biosciences , 561443, RRID: AB_10714629 , 1:100 for flow cytometry.

Techniques: Generated, Derivative Assay, Flow Cytometry, SYBR Green Assay, Recombinant, Software

Journal:

Article Title: Stem/progenitor cells from inflamed human dental pulp retain tissue regeneration potential

doi: 10.2217/rme.10.30

Figure Lengend Snippet: Density of marker expression in pulp.

Article Snippet: For immunostaining, primary antibodies used were nonimmune mouse IgG control (Vector Laboratories, Burlingame, CA, USA), mouse monoclonal anti-human STRO-1 (Invitrogen Corporation, Carlsbad, CA, USA), mouse monoclonal anti-human CD90 (BD Biosciences, San Jose, CA, USA), mouse monoclonal anti-human CD105 (Abcam, Cambridge, MA, USA) and mouse monoclonal anti-MUC18 (CD146; Invitrogen Corporation).

Techniques: Marker, Expressing, Staining